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JoVE Journal
Bioengineering

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甲基化特异性多路复用液滴PCR,使用聚合物液滴发生器设备进行血液诊断
 
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甲基化特异性多路复用液滴PCR,使用聚合物液滴发生器设备进行血液诊断

Article DOI: 10.3791/61421-v 09:05 min June 29th, 2020
June 29th, 2020

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Summary

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表观遗传标记用于白细胞(WBC)通过DNA甲基化模式的定量亚化。该协议提出了一种多路滴聚合酶链反应(mdPCR)方法,使用基于热塑性弹性体(TPE)的微流体器件进行液滴生成,从而实现WBC差分计数的精确和多路复用甲基化特异性目标定量。

Tags

本月在 JoVE, 问题 160, 甲基化, 多路复用液滴 PCR, 白细胞亚发, 表观遗传学, 液滴生成, 热塑性弹性体, 双硫酸盐处理 DNA
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