Journal
/
Bioengineering
/
Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

DOI:
10.3791/63984-v

•

08:55 min

•

July 06, 2022

•

, ,
1Department of Biomedical Engineering,University of Connecticut

Chapters

  • 00:04Introduction
  • 00:56Fabrication of the Janus Base Nano-Matrix (JBNm), Composed of Janus Base Nanotube (JBNt)/Matn1/Transforming Growth Factor Beta-1 (TGF-β1)
  • 01:41Observation of Specimens with Ultraviolet-Visible (UV-Vis) Spectroscopy
  • 03:12Absorption Spectra Measurement of Fluorescent-Labeled Proteins
  • 05:08Results: Layer-By-Layer Assembly of Janus Base Nano-Matrix Structure Scaffold for a Cartilage Tissue Construct
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the assembly of a layer-by-layer Janus base nano-matrix (JBNm) scaffold by adding Janus base nanotubes (JBNts), matrilin-3, and Transforming Growth Factor Beta-1 (TGF-β1) sequentially. The JBNm was fabricated and characterized; additionally, it displayed excellent bioactivity, encouraging cell functions such as adhesion, proliferation, and differentiation.

Tags

Keywords: Layer-by-layerJanus Base Nano-MatrixJBNmTGF-B1Matrilin-3Cartilage RegenerationGrowth Factor EncapsulationLocalized ChondrogenesisHomeostatic MicroenvironmentSpectrophotometryProtein Labeling

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63984?article_type=v

Microfluidic Chip Fabrication and Method to Detect Influenza
check_url/63984?article_type=v

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology
check_url/63984?article_type=v

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
check_url/63984?article_type=v

Fabrication and Characterization of a Conformal Skin-like Electronic System for Quantitative, Cutaneous Wound Management
check_url/63984?article_type=v

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
check_url/63984?article_type=v

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage
check_url/63984?article_type=v

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
check_url/63984?article_type=v

Fabrication and Characterization of Griffithsin-modified Fiber Scaffolds for Prevention of Sexually Transmitted Infections
check_url/63984?article_type=v

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure
check_url/63984?article_type=v

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Read Article