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免疫荧光DNA修复蛋白相互作用的可视化
JoVE Journal
Biology
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JoVE Journal Biology
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
DOI:

07:55 min

June 26, 2020

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Chapters

  • 00:04Introduction
  • 01:03Nuclear Extraction and Cell Fixation
  • 02:04Immunofluorescence Staining
  • 03:27Image Acquisition
  • 04:52Data Analysis
  • 05:30Results: Protein-protein Interaction at Sites of DNA Damage
  • 07:03Conclusion

Summary

Automatic Translation

在DNA损伤之后,人类细胞激活必要的修复途径,以恢复其基因组的完整性。在这里,我们描述了间接免疫荧光的方法,作为检测DNA修复蛋白,分析其空间和时间招募,并帮助查询DNA损伤点蛋白质-蛋白质相互作用的方法。

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