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Visualisering af DNA Reparation Proteiner Interaktion ved Immunofluorescence
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JoVE Journal Biology
Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Visualisering af DNA Reparation Proteiner Interaktion ved Immunofluorescence

DOI:
10.3791/61447-v

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07:55 min

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June 26, 2020

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,
1Department of Biochemistry and Structural Biology,University of Texas Health Science Center at San Antonio, 2Mays Cancer Center at UT Health San Antonio MD Anderson Cancer Center

Chapters

  • 00:04Introduction
  • 01:03Nuclear Extraction and Cell Fixation
  • 02:04Immunofluorescence Staining
  • 03:27Image Acquisition
  • 04:52Data Analysis
  • 05:30Results: Protein-protein Interaction at Sites of DNA Damage
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

Efter DNA-skader aktiverer humane celler vigtige reparationsveje for at genoprette integriteten af deres genom. Her beskriver vi metoden til indirekte immunfluorescens som et middel til at detektere DNA-reparationsproteiner, analysere deres rumlige og tidsmæssige rekruttering og hjælpe med at afhøre proteinproteininteraktion på steder med DNA-skader.

Tags

Keywords: DNA Repair ProteinsImmunofluorescenceProtein-protein InteractionsDNA DamageProtein FociColocalizationCell FixationPrimary AntibodySecondary Antibody

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