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Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay

Article DOI: 10.3791/61538-v • 09:57 min • August 6th, 2020
August 6th, 2020 •
1, 1,2,3, 1,4
1Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, 3Department of Immunology, Noguchi Memorial Institute for Medical Research, 4Centre for Medical Parasitology, Department of Infectious Diseases, Rigshospitalet

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Summary
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The overall goal of this protocol is to provide instruction on how to measure the capacity of antibodies present in sera or plasma of individuals, naturally exposed to Plasmodium falciparum infection, to opsonize and induce phagocytosis of the parasite-infected erythrocytes (IEs).

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Measuring Naturally Acquired Phagocytosis-Inducing Antibodies Plasmodium Falciparum Parasites Flow Cytometry-Based Assay Opsonize Phagocytosis Infected Erythrocytes Serum Individuals Parasite Infection Immunization Parasite Antigens THP-1 Cell Culture Experiment 25-centimeter Squared Culture Flask Concentration 96-well Plates FBS PBS Trophozoite-infected Erythrocytes Harvest Purification Parasite Culture Medium Parasitemia Magnetic Column Elute
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