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JoVE Journal Neuroscience
Derivazione, espansione, crioconservazione e caratterizzazione di cellule endoteliali microvascolari cerebrali da cellule staminali pluripotenti indotte dall'uomo
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Derivazione, espansione, crioconservazione e caratterizzazione di cellule endoteliali microvascolari cerebrali da cellule staminali pluripotenti indotte dall'uomo

Article DOI: 10.3791/61629-v • 08:04 min • November 19th, 2020
November 19th, 2020 •
1,2,3, 1,2,3,4, 1,3,4,5
1Center for Genomic Medicine, Massachusetts General Hospital, 2Department of Psychiatry, Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program, Broad Institute of MIT and Harvard, 4Department of Psychiatry, Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program, McLean Hospital

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Questo protocollo descrive in dettaglio un metodo adattato per derivare, espandere e crioconservare le cellule endoteliali microvascolari cerebrali ottenute differenziando le cellule staminali pluripotenti indotte dall'uomo e per studiare le proprietà della barriera eculare nel sangue in un modello ex vivo.

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Neuroscienze Numero 165 Cellule staminali pluripotenti indotte dall'uomo cellule endoteliali microvascolari cerebrali barriera emiencefalica resistenza elettrica trans-endoteliale attività del trasportatore microvascolare schizofrenia disturbo bipolare passaging espansione crioconservazione
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