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Untersuchung der Adipose-Gewebestruktur durch Methylsalicylat-Clearing und 3D-Bildgebung
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Untersuchung der Adipose-Gewebestruktur durch Methylsalicylat-Clearing und 3D-Bildgebung

Article DOI: 10.3791/61640-v • 10:10 min • August 19th, 2020
August 19th, 2020 •
1, 1, 2, 3, 1, 4, 4, 1, 1
1Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France

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Hier beschreiben wir eine einfache, kostengünstige und schnelle Clearing-Methode, um die 3D-Struktur von Maus- und menschlichem weiß-fettgewebe mithilfe einer Kombination von Markern zu lösen, um Vaskulatur, Kerne, Immunzellen, Neuronen und Lipid-Tröpfchen-Beschichtungsproteine durch fluoreszierende Bildgebung zu visualisieren.

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Biologie Ausgabe 162 Adipose-Gewebe Lichtung Lichtmikroskopie 3-D-Ganzesgewebe Adipositas Morphologie
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