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Visualisering av miniSOG Tagged DNA Repair Proteiner i kombinasjon med Electron Spektroskopiske Imaging (ESI)
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Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Visualisering av miniSOG Tagged DNA Repair Proteiner i kombinasjon med Electron Spektroskopiske Imaging (ESI)

DOI:
10.3791/52893-v

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13:06 min

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September 24, 2015

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, ,
1Department of Oncology, Faculty of Dentistry and Medicine,University of Alberta

Chapters

  • 00:05Title
  • 02:05Cell Culture and DNA Damage Induction
  • 03:13TEM Sample Preparation
  • 08:10TEM Imaging
  • 09:30Image Processing
  • 10:50Results: Electron Spectroscopic Imaging Describes Foci Changes Following DNA Damage
  • 12:21Conclusion

Summary

Automatic Translation

Automatic Translation

Electron spectroscopic imaging can image and distinguish nucleic acid from protein at nanometer resolution. It can be combined with the miniSOG system, which is able to specifically label tagged proteins in transmission electron microscopy samples. We illustrate the use of these technologies using double-strand break repair foci as an example.

Tags

Keywords: MiniSOGDNA Repair ProteinsElectron Spectroscopic Imaging (ESI)Transmission Electron MicroscopyProtein LocalizationDNA Double-strand Break RepairStructural Information

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