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Biochemistry
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PCR 突变体、克隆、表达、快速蛋白质纯化协议和色氨酸合成酶野生类型和突变形式的结晶
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Biochemistry
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JoVE Journal
Biochemistry
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Please note that all translations are automatically generated.
Click here for the English version.
PCR 突变体、克隆、表达、快速蛋白质纯化协议和色氨酸合成酶野生类型和突变形式的结晶
DOI:
10.3791/61839-v
•
09:31 min
•
September 26, 2020
•
Eduardo Hilario
,
Li Fan
,
Leonard J. Mueller
,
Michael F. Dunn
1
Department of Chemistry
,
University of California-Riverside
,
2
Department of Biochemistry
,
University of California-Riverside
Chapters
00:04
Introduction
00:44
Purification of Wild Type or Mutant Form of α
2
β
2
St
TS Complex
03:53
Optimized Crystallization for Wild Type and Mutant Form of the α
2
β
2
St
TS Comple
06:49
Results: Purification of the α- and β -Subunits of the Tryptophan Synthase and the Wild Type and Mutant α
2
β
2
St
TS Complexes
08:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一系列连续的方法来表达和净化
沙门氏菌色氨酸
合成酶,使该协议成为一天中净化蛋白质复合物的快速系统。覆盖的方法包括现场定向突变、
大肠杆菌
中的蛋白质表达、亲和色谱、凝胶过滤色谱和结晶。
Tags
PCR Mutagenesis
Cloning
Expression
Protein Purification
Tryptophan Synthase
Salmonella Typhimurium
Ammonium Sulfate Fractionation
Size Exclusion Chromatography
SDS-PAGE
Protein Concentration
Crystallization
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