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Pupal og voksen injeksjoner for RNAi og CRISPR Genredigering i Nasonia vitripennis
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Pupal og voksen injeksjoner for RNAi og CRISPR Genredigering i Nasonia vitripennis

Article DOI: 10.3791/61892-v • 08:41 min • December 4th, 2020
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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Her beskriver vi metoder for effektive pupal- og vokseninjeksjoner i Nasonia vitripennis som tilgjengelige alternativer til embryomikroinjeksjon, noe som muliggjør funksjonell analyse av gener av interesse ved hjelp av enten RNA-silencing via RNA-interferens (RNAi) eller gen knockout via CRISPR / Cas9 genomredigering.

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Biologi Utgave 166 Nasonia vitripennis CRISPR/Cas9 ReMOT BAPC-assistert CRISPR Vokseninjeksjoner RNAi
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