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Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal Chemistry
Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures
DOI:

10:10 min

December 01, 2020

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Chapters

  • 00:04Introduction
  • 00:56Crystal Harvesting and Mounting
  • 02:28Room Temperature Data Collection
  • 03:31Cryo Data Collection
  • 04:36Structure Refinement
  • 06:30Model Building
  • 07:45Results: Representative Neutron Crystallography Data Collection and Processing
  • 09:26Conclusion

Summary

Automatic Translation

Neutron protein crystallography is a structural technique that permits the localization of hydrogen atoms, thereby providing important mechanistic details of protein function. We present here the workflow for mounting a protein crystal, neutron diffraction data collection, structure refinement and analysis of the neutron scattering length density maps.

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