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睡眠美人转座转染人视网膜色素上皮细胞氧化应激的诱导与分析
 
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睡眠美人转座转染人视网膜色素上皮细胞氧化应激的诱导与分析

Article DOI: 10.3791/61957-v 10:00 min December 11th, 2020
December 11th, 2020

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Summary

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我们提出了一种开发和使用氧化应激模型的方案,方法是用H2O2处理视网膜色素上皮细胞,分析细胞形态,活力,密度,谷胱甘肽和UCP-2水平。它是研究转座子转染细胞分泌的蛋白质治疗神经视网膜变性的抗氧化作用的有用模型。

Tags

医学,第166期,眼基因治疗,年龄相关性黄斑变性,氧化应激损伤, 睡美人 转座子,非病毒基因递送,RPE细胞,PEDF,GM-CSF
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