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Højindhold Screening Differentiering og modning Analyse af Føtal og Voksen Neural Stamcelle-afledt Oligodendrocyte Precursor Cell Cultures
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Chapters
- 00:04Introduction
- 00:48Fetal Neural Stem Cell (NSC) Dissection and Isolation
- 02:18Adult NSC Culture
- 04:05Primary Neurosphere, Oligosphere, and Oligodendrocyte Precursor Cell (OPC) Differentiation
- 05:05Inflammation-Mediated Differentiation Block and Oxygen-Glucose Deprivation (OGD) Cell Death Induction
- 05:56High-Content Screening (HCS) Cell Viability, Lineage Composition, and Lineage-Specific Cell Death Analyses
- 07:18Results: Representative Oligosphere Differentiation Characterization
- 09:04Conclusion
Vi beskriver produktionen af blandede kulturer af astrocytter og oligodendrocyt prækursorceller afledt af føtale eller voksne neurale stamceller differentiere i modne oligodendrocytter, og in vitro modellering af skadelige stimuli. Koblingen med en cellebaseret screeningsteknik med højt indhold opbygger et pålideligt og robust lægemiddelscreeningssystem.
