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Journal / Genetics / Direkte omprogrammering av humane fibroblaster i…
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JoVE Journal Genetics
Direkte omprogrammering av humane fibroblaster i Myoblaster for å undersøke terapier for nevromuskulære lidelser
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Direkte omprogrammering av humane fibroblaster i Myoblaster for å undersøke terapier for nevromuskulære lidelser

Article DOI: 10.3791/61991-v • 10:28 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1, 2, 2,3,4,6, 5, 1,6, 1,6
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3The Heart Center, Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University

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Denne protokollen beskriver konvertering av hudfibroblaster til myoblaster og deres differensiering til myotubes. Cellelinjene er avledet fra pasienter med nevromuskulære lidelser og kan brukes til å undersøke patologiske mekanismer og for å teste terapeutiske strategier.

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Genetikk utgave 170 Omprogrammering hTERT MyoD fibroblast myoblast nevromuskulær lidelse terapitesting
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