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Høy oppløsning Whole Mount<em> In Situ</em> Hybridisering innenfor sebrafisk embryoer å studere Gene Expression og funksjon
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JoVE Journal Biology
High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Høy oppløsning Whole Mount<em> In Situ</em> Hybridisering innenfor sebrafisk embryoer å studere Gene Expression og funksjon

DOI:
10.3791/50644-v

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10:06 min

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October 19, 2013

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,
1Endocrine Research Laboratory and Department of Medicine,Royal Victoria Hospital, 2McGill University Health Centre Research Institute

Chapters

  • 00:05Title
  • 01:31Synthesis of Digoxygenin-labeled RNA Probes
  • 03:32Whole Mount in situ Hybridization
  • 06:55Representative in situ Hybridization Results
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Sebrafisk, en liten tropisk fisk, har blitt en populær modell for å studere gen-funksjon i løpet av virveldyr utvikling og sykdom. Den tidsmessige og romlige ekspresjon av målgener kan bestemmes ved<em> In situ</em> Hybridisering. Vår forbedrede Protokollen tillater påvisning av lave rikelig transkripter med lav ikke-spesifikk bakgrunn signal.

Tags

Gene ExpressionWhole Mount In Situ HybridizationWISHZebrafish EmbryosAntisense RNA ProbesDigoxigeninIn Vitro TranscriptionChromogenic SubstrateAlkaline PhosphataseDevelopmental Stage

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