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在健康和受伤的老鼠心脏中为三维纤维细胞组织进行精炼的清晰组织清除
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Medicine
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JoVE Journal Medicine
Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts
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在健康和受伤的老鼠心脏中为三维纤维细胞组织进行精炼的清晰组织清除

DOI:
10.3791/62023-v

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07:10 min

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May 16, 2021

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, , ,
1Department of Molecular Genetics, Biochemistry, and Microbiology,University of Cincinnati College of Medicine, 2Division of Molecular Cardiovascular Biology,Cincinnati Children’s Hospital Medical Center, 3Confocal Imaging Core,Cincinnati Children’s Hospital Medical Center

Chapters

  • 00:05Introduction
  • 00:38Adult Mouse Heart Clearing
  • 03:583D Cleared Heart Imaging
  • 04:53Results: Representative 3D Fibroblast Organization Visualization
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

开发并应用于成年小鼠心脏的组织清除方法。这种方法旨在清除密集的，自动荧光的心脏组织，同时保持标记成纤维细胞荧光归因于遗传报告器策略。

Tags

CLARITYFibroblastMouse Heart3D OrganizationInjuryFluorescenceHydrogelElectrophoresis

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