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High-Throughput Live Imaging af mikrokolonier til måling af heterogenitet i vækst og genekspression
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High-Throughput Live Imaging af mikrokolonier til måling af heterogenitet i vækst og genekspression

Article DOI: 10.3791/62038-v • 12:52 min • April 18th, 2021
April 18th, 2021 •
1, 1, 1, 1
1Center for Genomics and Systems Biology, Department of Biology, New York University

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Gær vækst fænotyper er præcist målt gennem meget parallelle time-lapse billeddannelse af immobiliserede celler vokser til mikrokolonier. Samtidig kan stresstolerance, proteinudtryk og proteinlokalisering overvåges og generere integrerede datasæt for at undersøge, hvordan miljømæssige og genetiske forskelle samt genekspressions heterogenitet blandt isogene celler modulerer vækst.

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Biologi vækstrate Saccharomyces cerevisiae nongenetisk heterogenitet høj-gennemløb mikroskopi naturlig variation
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