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High-Throughput Live Imaging af mikrokolonier til måling af heterogenitet i vækst og genekspression
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Biology
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JoVE Journal Biology
High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

High-Throughput Live Imaging af mikrokolonier til måling af heterogenitet i vækst og genekspression

DOI:
10.3791/62038-v

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12:52 min

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April 18, 2021

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, , ,
1Center for Genomics and Systems Biology, Department of Biology,New York University

Chapters

  • 00:00Introduction
  • 01:04Microscope Plate Setup
  • 03:16Yeast Dilution
  • 06:36Sonication (Optional)
  • 08:18Microscope Setup
  • 10:05Representative Results
  • 11:00Experimental Pitfalls
  • 12:22Conclusion

Summary

Automatic Translation

Automatic Translation

Gær vækst fænotyper er præcist målt gennem meget parallelle time-lapse billeddannelse af immobiliserede celler vokser til mikrokolonier. Samtidig kan stresstolerance, proteinudtryk og proteinlokalisering overvåges og generere integrerede datasæt for at undersøge, hvordan miljømæssige og genetiske forskelle samt genekspressions heterogenitet blandt isogene celler modulerer vækst.

Tags

High-throughputLive ImagingMicrocoloniesGrowthGene ExpressionHeterogeneityYeast CellsExperimental PlateConcanavalin AMicroscope PlateSterile Technique

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