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Karakterisering af en ny human organotypic retinal kultur teknik
JoVE Journal
Medicine
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JoVE Journal Medicine
Characterization of a Novel Human Organotypic Retinal Culture Technique
DOI:

05:51 min

June 09, 2021

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Chapters

  • 00:05Introduction
  • 00:53Media Preparation and Extraction of the Sandwich Retinal Explants from the Eyecup
  • 03:02Results: Observation of Cellular Integrity of Retinal Explants Using H&E, IHC and Analysis of Cytokine Release Using Magnetic Assay
  • 04:53Conclusion

Summary

Automatic Translation

Denne undersøgelse har til formål at udvikle en ny human organotypic retinal kultur (HORC) model, der forhindrer kompromittere retinal integritet under explant håndtering. Dette opnås ved at dyrke nethinden med den overliggende glaslegeme og det underliggende retinale pigment epitel-choroid (RPE-choroid) og sclera.

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