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活生物库的创建和维护 - 我们如何做到这一点
JoVE Journal
Cancer Research
Author Produced
This content is Free Access.
JoVE Journal
Cancer Research
Creation and Maintenance of a Living Biobank – How We Do It
Please note that all translations are automatically generated.
Click here for the English version.
活生物库的创建和维护 - 我们如何做到这一点
DOI:
10.3791/62065-v
•
13:08 min
•
April 10, 2021
•
Florian Bürtin
,
Stephanie Matschos
,
Friedrich Prall
,
Christina S. Mullins
,
Mathias Krohn
,
Michael Linnebacher
1
Department of General, Visceral, Vascular and Transplantation Surgery
,
University Medical Center Rostock, University of Rostock
,
2
Molecular Oncology and Immunotherapy, Department of General, Visceral, Vascular and Transplantation Surgery
,
University Medical Center Rostock
,
3
Institute of Pathology
,
University Medical Center Rostock
Chapters
00:00
Introduction
00:36
Sample Acquisition
02:41
Tissue Processing
05:43
Cell Culture
07:40
Generation of Patient-Derived Xenografts
11:16
Explantation of PDX
11:44
Representative Results
12:41
Conclusion
Summary
Automatic Translation
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Automatic Translation
在以下工作中,我们描述了建立结肠直肠癌和胰腺癌大型生物库所需的连续步骤。
Tags
Living Biobank
Patient-derived Models
Precision Medicine
Sample Acquisition
Serum Processing
PBL Isolation
Snap Freezing
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