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Immunology and Infection
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用于研究呼吸道病毒感染期间先天免疫细胞活化的非接触式共培养模型
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection
Please note that all translations are automatically generated.
Click here for the English version.
用于研究呼吸道病毒感染期间先天免疫细胞活化的非接触式共培养模型
DOI:
10.3791/62115-v
•
07:36 min
•
February 28, 2021
•
Zhe Zhang Ryan Lew*
1
,
Jing Liu*
1
,
Hsiao Hui Ong
,
Vivian Jiayi Tan
,
Annika Luukkainen
,
Yew Kwang Ong
4
,
Mark Thong
4
,
Kia Joo Puan
,
Vincent Tak Kwong Chow
6
,
Kai Sen Tan
2,6
,
De Yun Wang
6
1
Department of Otolaryngology, Yong Loo Lin School of Medicine
,
National University of Singapore
,
2
Department of Microbiology and Immunology, Yong Loo Lin School of Medicine
,
National University of Singapore
,
3
Haartman Institute, Medicum
,
University of Helsinki
,
4
Department of Otolaryngology, Head and Neck Surgery, National University Hospital
,
National University Health System
,
5
Agency for Science, Technology and Research (A*STAR)
,
Singapore Immunology Network (SIgN)
,
6
NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine
,
National University of Singapore
Chapters
00:04
Introduction
00:50
hNEC Viral Infection and hNEC + PBMCs Co-Culture
03:30
Harvesting of PBMCs 48/72 h Post-Viral Infection
05:31
Results: Activation of T Cells Following Influenza Infection
06:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议详细研究了病毒感染的鼻上皮细胞与先天细胞活化之间的早期相互作用。免疫细胞的单个亚群可以根据其对病毒感染的反应而激活来区分。然后可以进一步研究它们以确定它们对早期抗病毒反应的影响。
Tags
Contact-free Co-culture
Innate Immune Cell Activation
Respiratory Virus Infection
Epithelial Cells
Innate T Cells
Influenza
Anti-viral Therapy
Human Nasal Epithelial Cells
Multiplicity Of Infection
Peripheral Blood Mononuclear Cells
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