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端粒上的化学分化诱导蛋白凝结物
JoVE Journal
Biology
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JoVE Journal Biology
Chemical Dimerization-Induced Protein Condensates on Telomeres
DOI:

08:52 min

April 12, 2021

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Chapters

  • 00:04Introduction
  • 00:37Dimerization on Telomeres
  • 01:11Immunofluorescence (IF)
  • 03:02Fluorescence In Situ Hybridization (FISH)
  • 04:14Imaging
  • 06:23Results: Chemical Dimerization to Induce Chromatin-associated Condensates
  • 08:06Conclusion

Summary

Automatic Translation

该协议说明了化学诱导的蛋白质变暗系统,在染色质上产生凝结物。 用化学调光剂在端粒上形成前列腺白血病(PML)核体。滴生长、溶解、定位和组成通过活细胞成像、免疫荧光 (IF) 和荧光就地杂交 (FISH) 进行监测。

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