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Biochemistry

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JoVE Journal Biochemistry
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Article DOI: 10.3791/62177-v • 08:49 min • March 14th, 2021
March 14th, 2021 •
*1, *1, 1, 1
1Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire
* These authors contributed equally

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Summary
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Here, we describe protocols using fluorescent lipid sensors and liposomes to determine whether a protein extracts and transports phosphatidylserine or phosphatidylinositol 4-phosphate in vitro.

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Fluorescence-based Measurements Phosphatidylserine Phosphatidylinositol 4-phosphate Exchange Between Membranes Protein Transfer Synthetic Membrane Lipid Distribution Eukaryotic Cells Fluorescence Cells Liposomes Cellular Biology Real-time Measurements Lipid Transfer Maud Magdeleine HEPES Potassium Acetate Buffer HKM Buffer PC Liposomes PS Liposomes PI(4)P Liposomes Rotary Evaporator
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