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Toxicity Screens in Human Retinal Organoids for Pharmaceutical Discovery
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Bioengineering
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JoVE Journal Bioengineering
Toxicity Screens in Human Retinal Organoids for Pharmaceutical Discovery

Toxicity Screens in Human Retinal Organoids for Pharmaceutical Discovery

DOI:
10.3791/62269-v

•

07:45 min

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March 04, 2021

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, , ,
1Lowy Medical Research Institute, 2The Scripps Research Institute

Chapters

  • 00:04Introduction
  • 00:47Deoxysphinganine (deoxySA) Treatment
  • 02:23Organoid Slice Imaging
  • 03:35Cell Death Quantification
  • 05:07Drug Treatment
  • 06:06Results: Representative Effects of DeoxySA on Retinal Organoid Cell Death
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a step-by-step protocol to generate mature human retinal organoids and utilize them in a photoreceptor toxicity assay to identify pharmaceutical candidates for the age-related retinal degenerative disease macular telangiectasia type 2 (MacTel).

Tags

Keywords: Retinal OrganoidsPharmaceutical DiscoveryMetabolic Retinal DiseasesMacTelFenofibrateRetinal DegenerationToxicity ScreensDeoxySAPhotoreceptorCell DeathPreclinical AssayCell CultureRetinal Differentiation

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