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Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
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Cancer Research
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JoVE Journal Cancer Research
Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

DOI:
10.3791/58386-v

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08:38 min

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October 04, 2018

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1Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership,University of Oslo, 2K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy,University of Oslo

Chapters

  • 00:04Title
  • 00:32Preparation, Stimulation, and Fixation of Cells
  • 01:50Fluorescent Cell Barcoding
  • 03:30Cell Permeabilization and Intracellular Antigen Staining
  • 06:12Results: Phospho Flow Cytometry Analysis with Fluorescent Cell Barcoding
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a protocol for medium- to high-throughput analysis of protein phosphorylation events at the cellular level is presented. Phospho flow cytometry is a powerful approach to characterize signaling aberrations, identify and validate biomarkers, and assess pharmacodynamics.

Tags

Keywords: Phospho Flow CytometryFluorescent Cell BarcodingSingle Cell Signaling AnalysisBiomarker DiscoveryCell BiologyImmunologySignaling PathwaysStimuliDrugsHigh ThroughputRPMI 16/40 MediumFixed BufferAnti-IgMPBSBarcoding ReagentsPerm BufferCompensation Control

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