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Værktøjer til realtidsvurdering af en Pseudomonas aeruginosa-infektionsmodel
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model
DOI:

07:39 min

April 06, 2021

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Chapters

  • 00:05Introduction
  • 00:34Synthetic Cystic Fibrosis Medium (SCFM2) Preparation
  • 01:03Bacterial Cell Suspension Preparation
  • 02:36Pseudomonas aeruginosa Aggregate Visualization and Isolation
  • 04:48Image Analysis
  • 06:15Results: Analysis of Pseudomonas aeruginosa Aggregates
  • 07:10Conclusion

Summary

Automatic Translation

Syntetisk cystisk fibrose spyt medium (SCFM2) kan udnyttes i kombination med både konfokal laser scanning mikroskopi og fluorescens-aktiveret celle sortering til at observere bakterielle aggregater ved høj opløsning. Dette dokument beskriver metoder til at vurdere samlede populationer under antimikrobiel behandling som en platform for fremtidige undersøgelser.

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