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Værktøjer til realtidsvurdering af en Pseudomonas aeruginosa-infektionsmodel
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Immunology and Infection
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JoVE Journal Immunology and Infection
Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model

Værktøjer til realtidsvurdering af en Pseudomonas aeruginosa-infektionsmodel

DOI:
10.3791/62420-v

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07:39 min

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April 06, 2021

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1Department of Molecular Medicine,Morsani College of Medicine, University of South Florida

Chapters

  • 00:05Introduction
  • 00:34Synthetic Cystic Fibrosis Medium (SCFM2) Preparation
  • 01:03Bacterial Cell Suspension Preparation
  • 02:36Pseudomonas aeruginosa Aggregate Visualization and Isolation
  • 04:48Image Analysis
  • 06:15Results: Analysis of Pseudomonas aeruginosa Aggregates
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

Syntetisk cystisk fibrose spyt medium (SCFM2) kan udnyttes i kombination med både konfokal laser scanning mikroskopi og fluorescens-aktiveret celle sortering til at observere bakterielle aggregater ved høj opløsning. Dette dokument beskriver metoder til at vurdere samlede populationer under antimikrobiel behandling som en platform for fremtidige undersøgelser.

Tags

Pseudomonas AeruginosaInfection ModelReal-time AssessmentAntibiotic ResistanceMucinSynthetic Cystic Fibrosis Sputum MediumConfocal Laser Scanning MicroscopeBacterial AggregatesOptical DensityLog PhasePBS WashStatic Incubation

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