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使用夹层冷冻装置快速冷冻,在电子显微镜下对生物标本进行良好的超微结构保存
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用夹层冷冻装置快速冷冻,在电子显微镜下对生物标本进行良好的超微结构保存
DOI:
10.3791/62431-v
•
09:03 min
•
July 19, 2021
•
Masashi Yamaguchi
,
Azusa Takahashi-Nakaguchi
,
Katsuyuki Uematsu
,
Masaki Taguchi
,
Michiyo Sato-Okamoto
,
Hiroji Chibana
1
Medical Mycology Research Center
,
Chiba University
,
2
Marine Works Japan Ltd.
Chapters
00:04
Introduction
00:48
Rapid Freezing of Cell Suspensions for Freeze-Substitution
07:19
Results: Ultrastructural Preservation of Biological Specimens in Electron Microscopy Using Sandwich Freezing Device
08:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们将展示如何使用三明治冷冻装置快速冷冻生物标本,包括细菌,酵母,培养细胞,分离细胞,动物和人体组织以及病毒。我们还展示了如何在快速冷冻后为超薄切片制备试样。
Tags
Rapid Freezing
Sandwich Freezing Device
Ultrastructural Preservation
Biological Specimens
Electron Microscopy
Cell Fixation
Ice Crystal Formation
Copper Discs
Glow Discharge
Centrifugation
Propane Freezing
Glutaraldehyde Fixation
Freeze-substitution
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