JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
電子顕微鏡での生体試料の良好な超構造保存のためのサンドイッチ凍結装置を用いた急速凍結
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
電子顕微鏡での生体試料の良好な超構造保存のためのサンドイッチ凍結装置を用いた急速凍結
DOI:
10.3791/62431-v
•
09:03 min
•
July 19, 2021
•
Masashi Yamaguchi
,
Azusa Takahashi-Nakaguchi
,
Katsuyuki Uematsu
,
Masaki Taguchi
,
Michiyo Sato-Okamoto
,
Hiroji Chibana
1
Medical Mycology Research Center
,
Chiba University
,
2
Marine Works Japan Ltd.
Chapters
00:04
Introduction
00:48
Rapid Freezing of Cell Suspensions for Freeze-Substitution
07:19
Results: Ultrastructural Preservation of Biological Specimens in Electron Microscopy Using Sandwich Freezing Device
08:03
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ここでは、細菌、酵母、培養細胞、単離細胞、動物およびヒト組織、ウイルスを含む生物学的標本の迅速凍結にサンドイッチ凍結装置を使用する方法を示す。また、急速凍結後の超薄切片用試料の調製方法もご紹介します。
Tags
Rapid Freezing
Sandwich Freezing Device
Ultrastructural Preservation
Biological Specimens
Electron Microscopy
Cell Fixation
Ice Crystal Formation
Copper Discs
Glow Discharge
Centrifugation
Propane Freezing
Glutaraldehyde Fixation
Freeze-substitution
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
凍結ヒトES細胞
Flashは、E12.5マウス脳の凍結とCryosectioning
トランスポートのための凍結、融解、およびパッケージング細胞
ヒト胚性幹細胞を凍結融解
磁気変調バイオセンシングシステムを用いた生物学的アッセイの迅速な均質な検出
組織トリアージや骨格筋疾患のモデルのために凍結
生物学電子顕微鏡でのフリーズフラクチャー/フリーズエッチングの基本技術要素
タンデム高圧凍結および透過型電子顕微鏡のための植物組織の急速凍結置換
プランジ凍結:透過型電子顕微鏡での懸濁細胞の超微細構造と免疫学的研究のためのツール
マリンTubewormでライブ光学および電子顕微鏡技術を用いた石灰化イベントのキャラクタリゼーション
Read Article