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Journal
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Immunology and Infection
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猪肠粘膜上皮中靶向氨基肽酶N的单克隆抗体的生产
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
Please note that all translations are automatically generated.
Click here for the English version.
猪肠粘膜上皮中靶向氨基肽酶N的单克隆抗体的生产
DOI:
10.3791/62437-v
•
09:45 min
•
May 18, 2021
•
Pengpeng Xia
2,3
,
Siqi Lian
2,3
,
Yunping Wu
2,3
,
Li Yan
2,3
1
College of Veterinary Medicine (Institute of comparative medicine)
,
Yangzhou University
,
2
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses
,
3
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China
,
Yangzhou University
Chapters
00:04
Introduction
01:04
Hybridoma Technology to Produce Monoclonal Antibodies (mAbs) Against Aminopeptidase N (APN)
04:20
Expression of Recombinant Antibodies (rAbs) Against APN
06:25
Results: Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
09:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
pIRES2-ZSGreen1-rAbs-APN-CHO细胞表达的重组抗体蛋白和采用传统杂交瘤技术生产的单克隆抗体可以识别并与猪氨基肽酶N(APN)蛋白结合。
Tags
Monoclonal Antibodies
Aminopeptidase N
Porcine Intestinal Mucosal Epithelium
Recombinant Antibody Production
Antibody Drug Conjugates
Hybridization
SP20 Myeloma Cells
Peritoneal Macrophages
Polyethylene Glycol
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