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Journal
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Medicine
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温缺血/再灌注损伤的大鼠肺移植模型:改善结局的优化
JoVE Journal
Medicine
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JoVE Journal
Medicine
A Rat Lung Transplantation Model of Warm Ischemia/Reperfusion Injury: Optimizations to Improve Outcomes
Please note that all translations are automatically generated.
Click here for the English version.
温缺血/再灌注损伤的大鼠肺移植模型:改善结局的优化
DOI:
10.3791/62445-v
•
07:37 min
•
October 28, 2021
•
Yong Gyu Lee
2
,
Jung-Lye Kim
2
,
Andre F. Palmer
,
Brenda F. Reader
,
Jianjie Ma
,
Sylvester M. Black
4
,
Bryan A. Whitson
2
1
Department of Surgery Division of Cardiac Surgery
,
The Ohio State University Wexner Medical Center
,
2
The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory
,
The Ohio State University College of Medicine
,
3
William G. Lowrie Department of Chemical and Biomolecular Engineering, College of Engineering
,
The Ohio State University
,
4
Comprehensive Transplant Center
,
The Ohio State University Wexner Medical Center
Chapters
00:04
Introduction
00:45
Initial Setup
02:53
Donor Lung Warm Ischemia and Procurement
04:14
Recipient Rat Preparation and Anastomoses
06:12
Reperfusion
06:35
Results: The Wet‐To‐Dry Weight Ratio of The Lobes
06:55
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了对大鼠肺移植模型的优化,以改善结果。我们提供基于体重的袖带尺寸指南、确定
第 4 肋
间隙的测量策略以及伤口闭合和 BAL(支气管肺泡灌洗)液体和组织收集的方法。
Tags
Rat Lung Transplantation
Warm Ischemia/reperfusion Injury
Surgical Technique
Cuffing Method
Angio-catheter
Thoracic Incision
Endotracheal Intubation
Heparin Administration
Molecular Mechanisms
Therapeutic Interventions
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