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Screening peptider, der aktiverer MRGPRX2 ved hjælp af manipulerede HEK-celler
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
DOI:

12:38 min

November 06, 2021

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Chapters

  • 00:03Introduction
  • 02:08Design and Development of Peptide Library
  • 02:56In vitro Cell Culture
  • 04:42Fura-2 Calcium Assay
  • 05:35Cell Activation and Fluorescent Reading
  • 09:28Data Analysis
  • 09:38Representative Results
  • 12:13Conclusion

Summary

Automatic Translation

Teknikker til generering af et bibliotek af korte peptider, der kan aktivere mastceller via MRGPRX2-receptoren, beskrives. Tilknyttede teknikker er nemme, billige, og kan udvides til andre celle receptorer.

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