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Journal
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Immunology and Infection
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使用基于HiBiT的生物报告器检测SARS-CoV-2受体结合域抗体
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
Please note that all translations are automatically generated.
Click here for the English version.
使用基于HiBiT的生物报告器检测SARS-CoV-2受体结合域抗体
DOI:
10.3791/62488-v
•
07:44 min
•
August 12, 2021
•
Reza Rezaei
2
,
Abera Surendran
2
,
Ragunath Singaravelu
2
,
Taylor R. Jamieson
2
,
Parisa Taklifi
,
Joanna Poutou
2
,
Taha Azad
2
,
Carolina S. Ilkow
2
1
Ottawa Hospital Research Institute
,
2
Department of Biochemistry, Microbiology and Immunology
,
University of Ottawa
,
3
Department of Biotechnology
,
University of Tehran
Chapters
00:05
Introduction
00:50
Production and Evaluation of the HiBiT-RBD Bioreporter
03:47
Detecting Anti-RBD Antibody with a Fast and Sensitive Assay
04:53
High-Throughput Detection of the SARS-CoV-2-Specific Antibodies from Patient Serum Samples
06:05
Results: SARS-CoV-2 Antibody Detection Using HiBiT-Based Bioreporter
07:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
概述的方案描述了生产HiBiT受体结合结构域蛋白复合物的程序及其在快速灵敏地检测SARS-CoV-2抗体中的应用。
Tags
SARS-CoV-2
Receptor-binding Domain
Antibody Detection
HiBiT-based Bioreporter
COVID-19 Research
High-throughput Assay
Cell Culture
Transfection
Passive Lysis Buffer
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