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Bioengineering
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通过红外激光辅助微模式控制细胞几何
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Bioengineering
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JoVE Journal
Bioengineering
Control of Cell Geometry through Infrared Laser Assisted Micropatterning
Please note that all translations are automatically generated.
Click here for the English version.
通过红外激光辅助微模式控制细胞几何
DOI:
10.3791/62492-v
•
11:04 min
•
July 10, 2021
•
Shuying Yang
,
Chen Tuo
,
Ernest Iu
,
Sergey V. Plotnikov
1
Department of Cell and Systems Biology
,
University of Toronto
Chapters
00:00
Introduction
01:16
Coating Coverslips with PVA
02:09
Setting Up Optical Configurations
06:47
Mounting the Coverslip and Setting Up the Macro
08:45
Results
10:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里提出的协议使微型模式能够自动制造,使细胞形状标准化,以研究哺乳动物细胞中的细胞骨结构。这种用户友好型技术可以与市售成像系统建立,不需要标准细胞生物学实验室无法使用的专用设备。
Tags
Micropatterning
Cell Geometry
Infrared Laser
Automated Workflow
Image Processing
Protein Distribution
PVA Solution
Coverslip Spinner
Multiphoton Imaging System
Laser-assisted Patterning
Cell Shape
Intercellular Machines
Automated Micropatterning
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