The generation of aligned myocardial tissue is a key requirement for adapting the recent advances in stem cell biology to clinically useful purposes. Herein we describe a microcontact printing approach for the precise control of cell shape and function. Using highly purified populations of embryonic stem cell derived cardiac progenitors, we then generate anisotropic functional myocardial tissue.
Atmanli, A., Domian, I. J. Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing. J. Vis. Exp. (73), e50288, doi:10.3791/50288 (2013).