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朗根多夫方法的修改，用于从成年小鼠中同时分离心房和心室肌细胞
JoVE Journal
Medicine
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JoVE Journal Medicine
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
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朗根多夫方法的修改，用于从成年小鼠中同时分离心房和心室肌细胞

DOI:
10.3791/62514-v

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06:27 min

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May 13, 2021

•

, , , , , , , , , , ,
1Department of Cardiology, Bejing Anzhen Hospital,Capital Medical University, 2Department of Cardiology,Bejing Chuiyangliu Hospital

Chapters

  • 00:05Introduction
  • 00:38Heart Excision and Aortic Cannulation
  • 02:38Heart Perfusion
  • 03:37Cell Isolation and Calcium Reintroduction
  • 04:46Results: Survival Rates and Quality of the Isolated Cardiac Myocytes
  • 06:00Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了Langendorff方法的修改，包括主动脉插管的深度，以便同时从成年小鼠中分离心房和心室肌细胞。

Tags

Langendorff MethodAtrial MyocytesVentricular MyocytesAdult MiceSimultaneous IsolationHeart DiseaseCellular MechanismsEuthanasiaAortic CannulationLangendorff ApparatusEnzymatic DigestionCell Isolation

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