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朗根多夫方法的修改,用于从成年小鼠中同时分离心房和心室肌细胞
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
DOI:

06:27 min

May 13, 2021

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Chapters

  • 00:05Introduction
  • 00:38Heart Excision and Aortic Cannulation
  • 02:38Heart Perfusion
  • 03:37Cell Isolation and Calcium Reintroduction
  • 04:46Results: Survival Rates and Quality of the Isolated Cardiac Myocytes
  • 06:00Conclusion

Summary

Automatic Translation

该协议描述了Langendorff方法的修改,包括主动脉插管的深度,以便同时从成年小鼠中分离心房和心室肌细胞。

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