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成体マウスからの心房と心室筋細胞の同時分離のためのランゲンドルフ法の改変
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
Please note that all translations are automatically generated.
Click here for the English version.
成体マウスからの心房と心室筋細胞の同時分離のためのランゲンドルフ法の改変
DOI:
10.3791/62514-v
•
06:27 min
•
May 13, 2021
•
Kui Wu
,
Lin-Ling Li
,
Yu-Kun Li
,
Xiao-Dong Peng
,
Meng-Xia Zhang
,
Ke-Sen Liu
,
Xue-Si Wang
,
Jia-Xue Yang
,
Song-Nan Wen
,
Yan-Fei Ruan
,
Nian Liu
,
Rong Bai
1
Department of Cardiology, Bejing Anzhen Hospital
,
Capital Medical University
,
2
Department of Cardiology
,
Bejing Chuiyangliu Hospital
Chapters
00:05
Introduction
00:38
Heart Excision and Aortic Cannulation
02:38
Heart Perfusion
03:37
Cell Isolation and Calcium Reintroduction
04:46
Results: Survival Rates and Quality of the Isolated Cardiac Myocytes
06:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、成体マウスからの心房および心室筋細胞の同時分離のための大歯カンヌレーションの深さを含むランゲンドルフ法の修飾を記述する。
Tags
Langendorff Method
Atrial Myocytes
Ventricular Myocytes
Adult Mice
Simultaneous Isolation
Heart Disease
Cellular Mechanisms
Euthanasia
Aortic Cannulation
Langendorff Apparatus
Enzymatic Digestion
Cell Isolation
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