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Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
JoVE Journal
Biology
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JoVE Journal Biology
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
DOI:

14:30 min

August 07, 2021

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Chapters

  • 00:00Introduction
  • 01:17Background
  • 02:12Methods: Immunophenotyping
  • 03:50Results
  • 06:58Relevance
  • 07:42Methods: Cell Sorting
  • 12:27Results and Post-Sort Analysis
  • 12:53Limitations and Perspective

Summary

Automatic Translation

Here, we present an immunophenotyping strategy for the characterization of megakaryocyte differentiation, and show how that strategy allows the sorting of megakaryocytes at different stages with a fluorescence-activated cell sorter. The methodology can be applied to human primary tissues, but also to megakaryocytes generated in culture in vitro.

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