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ヒトの一次源または造血前駆体からの分化 インビトロ からのヒトMKの免疫フェノタイピングと細胞選別
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated <em>In Vitro</em> from Hematopoietic Progenitors
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Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

ヒトの一次源または造血前駆体からの分化 インビトロ からのヒトMKの免疫フェノタイピングと細胞選別

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6,486 Views

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14:30 min

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August 07, 2021

DOI:

10.3791/62569-v

DOI:
10.3791/62569-v

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14:30 min
August 07, 2021

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12 Views
, , , , , , ,
1Platelet Research Lab,Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 2Flow Cytometry and Cell Sorting Platform (ISPA), 3Clinical Diagnosis Laboratory – Dept. of Hematology,Hospital Universitario Central de Asturias (HUCA), 4Department of Hematology,Hospital Universitario Severo Ochoa (HUSO), 5Department of Hematology,Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), 6Dept. of Medicine,University of Oviedo

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ISPAアストゥリアス公国健康研究所の血小板研究所のアンドレア・アセビス・ウエルタ博士研究員です。これはオビエド、スペインに位置しています。こんにちは.こんにちは.私はローラ・グティです

Summary

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ここでは、巨核球分化の特徴付けに関する免疫型付け戦略を紹介し、その戦略が蛍光活性化細胞選別機を用いて異なる段階で巨核球を選別する方法を示す。この方法論はヒトの一次組織に適用できるが、 インビトロの培養で生成された巨核球にも適用できる。

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Cite this Article

Acebes-Huerta, A., Martínez-Botía, P., Martín Martín, C., Bernardo, Á., Rodríguez, A. R., Vicente-Ayuso, M. C., Benavente Cuesta, C., Gutiérrez, L. Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors. J. Vis. Exp. (174), e62569, doi:10.3791/62569 (2021).

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