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Montering av cellemikrokering støttede og suspenderte Lipid Bilayer-modeller for studiet av molekylære interaksjoner
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
DOI:

12:18 min

August 03, 2021

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Chapters

  • 00:04Introduction
  • 00:33Developing and Characterizing Uni-Lipid Vesicles
  • 04:33Forming a Uni-Lipid Supported Lipid Bilayer Using QCM-D
  • 07:29Forming a Suspended Lipid Bilayer
  • 08:05Developing Multi-Lipid Cell Mimicking Vesicles and Bilayers
  • 08:48Molecule Interaction Studies with Uni-Lipid and Multi-Lipid Supported Bilayers
  • 09:29Molecule Interaction Studies with Uni-Lipid and Multi-Lipid Suspended Bilayers
  • 10:15Results: Development of Uni- and Multi-Lipid Cell Mimicking Vesicles and Bilayers
  • 11:51Conclusion

Summary

Automatic Translation

Denne protokollen beskriver dannelsen av celle som etterligner uni-lipid og multi-lipid vesicles, støttet lipid bilayers, og suspendert lipid bilayers. Disse in vitro-modellene kan tilpasses for å inkorporere en rekke lipidtyper og kan brukes til å undersøke ulike molekyl- og makromolekylinteraksjoner.

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