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RNA-seq数据中替代剪接和聚腺苷酸化的鉴定

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Biology

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JoVE Journal Biology

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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RNA-seq数据中替代剪接和聚腺苷酸化的鉴定

DOI:

10.3791/62636-v

•

08:35 min

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June 24, 2021

•

Gunjan Dixit, Ying Zheng, Brian Parker, Jiayu Wen

¹Department of Genome Sciences, The John Curtin School of Medical Research,The Australian National University, ²Department of Biology,New York University

Chapters

00:05Introduction
00:51Alternative Splicing (AS) Analysis Using RNA-Seq
06:02Results: Alternative Splicing and Polyadenylation in RNA-Seq Data
07:46Conclusion

Summary

Automatic Translation

选择性剪接（AS）和替代聚腺苷酸化（APA）扩大了转录本亚型及其产物的多样性。在这里，我们描述了生物信息学协议，以分析批量RNA-seq和3'末端测序测定，以检测和可视化不同实验条件下变化的AS和APA。

Tags

Alternative Splicing Polyadenylation RNA-seq Differential Splicing Differential Exon Expression Limma EdgeR Voom DiffSplice

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