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Journal / Biology / 5-Ethynyl-2'-Deoxyuridin/Fosfo-Histone H3…
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5-Ethynyl-2'-Deoxyuridin/Fosfo-Histone H3 Dobbeltmærkning protokol for Cell Cycle Progression Analyse i Drosophila neurale stamceller
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5-Ethynyl-2'-Deoxyuridin/Fosfo-Histone H3 Dobbeltmærkning protokol for Cell Cycle Progression Analyse i Drosophila neurale stamceller

Article DOI: 10.3791/62642-v • 09:34 min • May 4th, 2021
May 4th, 2021 •
1,2, 1
1Cell Biology, University of Bern, 2Department of life sciences and medicine, University of Luxembourg

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Cellecyklusanalyse med 5-ethynyl-2'-deoxyuridin (EdU) og fosfo-histone H3 (pH3) mærkning er en flertrinsprocedure, der kan kræve omfattende optimering. Her præsenterer vi en detaljeret protokol, der beskriver alle trin for denne procedure, herunder billedanalyse og kvantificering for at skelne celler i forskellige cellecyklusfaser.

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Biologi udgave 171
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