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Chapters
- 00:04Introduction
- 00:40Dissection of Third Instar Larval Brains and 5-ethynyl-2 -deoxyuridine (EdU) Incorporation
- 02:23EdU Detection, DNA Staining, and Mounting
- 04:29Imaging
- 05:52Image Analysis
- 08:06Results: EdU and pH3 Dual-labeling for Cell Cycle Progression Analysis in Drosophila Neural Stem Cells
- 09:01Conclusion
Cellecyklusanalyse med 5-ethynyl-2'-deoxyuridin (EdU) og fosfo-histone H3 (pH3) mærkning er en flertrinsprocedure, der kan kræve omfattende optimering. Her præsenterer vi en detaljeret protokol, der beskriver alle trin for denne procedure, herunder billedanalyse og kvantificering for at skelne celler i forskellige cellecyklusfaser.
