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Biochemistry
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检测蛋白质低洼构象状态的高压NMR实验
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Biochemistry
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JoVE Journal
Biochemistry
High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
Please note that all translations are automatically generated.
Click here for the English version.
检测蛋白质低洼构象状态的高压NMR实验
DOI:
10.3791/62701-v
•
04:37 min
•
June 29, 2021
•
Trang T. Nguyen
,
Steven Siang
,
Julien Roche
1
Department of Chemistry
,
Iowa State University
,
2
Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology
,
Iowa State University
Chapters
00:05
Introduction
00:34
Sample Preparation and High-Pressure Cell Assembly
02:03
Recording High-Pressure NMR Experiments
02:32
Results: Unfolding of hnRNPA1 RRM2 Domain Under Pressure
04:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们详细描述了组装高压电池所需的步骤,建立并记录了高压NMR实验,最后分析了压力下的峰值强度和化学移位变化。这些实验可以为蛋白质的折叠途径和结构稳定性提供有价值的见解。
Tags
High-pressure NMR
Protein Conformational States
Pressure Perturbation
Glass Cavities
Void Volumes
Nitrogen-15-labeled Sample
Mineral Oil
Transmission Liquid
Pressure Cell Assembly
Transverse Relaxation Optimized Spectroscopy
Hetero Single-quantum Coherence Spectroscopy
Folding/unfolding Rates
RRM2
Heterogeneous Nuclear Ribonucleoprotein A1
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