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Biology
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快速、经济、简单地生产无细菌细胞裂解物
JoVE Journal
Biology
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JoVE Journal
Biology
Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate
Please note that all translations are automatically generated.
Click here for the English version.
快速、经济、简单地生产无细菌细胞裂解物
DOI:
10.3791/62753-v
•
06:03 min
•
October 29, 2021
•
Robert M. Cooper*
1
,
Taishi Tonooka*
1,2
,
Andriy Didovyk*
1,3
,
Jeff Hasty
4,5
1
Biocircuits Institute
,
University of California, San Diego
,
2
Present address
,
Kyoto Institute of Technology
,
3
Present address
,
Vertex Pharmaceuticals
,
4
Department of Bioengineering
,
University of California, San Diego
,
5
Molecular Biology Section
,
University of California, San Diego
Chapters
00:04
Introduction
00:38
Cell Preparation
02:05
Lysate Preparation
04:13
Cell-Free Gene Expression
04:47
Results: Green Fluorescent Protein (GFP) Expression Using the Autolysate
05:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了一种快速而简单的方法,使用工程化的
大肠杆菌
菌株生产细菌裂解物以实现无细胞基因表达,并且只需要标准的实验室设备。
Tags
Bacterial Cell-free Lysate
Cell-free Gene Expression
Autolysis E. Coli
LB Agar
Ampicillin
Starter Culture
2xYTPG Medium
Optical Density
Centrifugation
S30A Buffer
Dithiothreitol
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