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JoVE Journal Neuroscience
Real-time fluorescerende meting van synaptische functies in modellen van amyotrofische laterale sclerose
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Real-time fluorescerende meting van synaptische functies in modellen van amyotrofische laterale sclerose

Article DOI: 10.3791/62813-v • 08:59 min • July 16th, 2021
July 16th, 2021 •
*1, 1, 1, *1
1Jefferson Weinberg ALS Center, Thomas Jefferson University
* These authors contributed equally

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Twee gerelateerde methoden worden beschreven om subcellulaire gebeurtenissen te visualiseren die nodig zijn voor synaptische transmissie. Deze protocollen maken de real-time monitoring van de dynamiek van presynaptische calciuminstroom en synaptische vesikelmembraanfusie mogelijk met behulp van live-cell imaging van in vitro gekweekte neuronen.

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Neurowetenschappen Nummer 173
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