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Real-time fluorescerende meting van synaptische functies in modellen van amyotrofische laterale sclerose
 
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Real-time fluorescerende meting van synaptische functies in modellen van amyotrofische laterale sclerose

Article DOI: 10.3791/62813-v
July 16th, 2021

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Summary July 16th, 2021

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Twee gerelateerde methoden worden beschreven om subcellulaire gebeurtenissen te visualiseren die nodig zijn voor synaptische transmissie. Deze protocollen maken de real-time monitoring van de dynamiek van presynaptische calciuminstroom en synaptische vesikelmembraanfusie mogelijk met behulp van live-cell imaging van in vitro gekweekte neuronen.

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