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JoVE Journal
Immunology and Infection

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Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
 

Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology

Article DOI: 10.3791/62834-v
May 31st, 2022

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Summary May 31st, 2022

This protocol has been established to culture tetrahydrobiopterin (BH4)- and inducible nitric oxide synthase (iNOS)-deficient primary murine macrophages to study NO-redox biology. The study focuses on reducing potential contamination of BH4 and other artifacts found in traditional isolation and culture methods which may confound experimental outcomes and interpretation of results.

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