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An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
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1Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James’s Hospital, Trinity College Dublin,The University of Dublin, 2School of Pharmacy and Biomolecular Sciences,Royal College of Surgeons in Ireland (RCSI), 3SFI Advanced Materials and Bioengineering Research (AMBER) Centre,RCSI & TCD, 4SFI Centre for Research in Medical Devices (CURAM), RCSI,Dublin and National University of Ireland
Chapters
- 00:04Introduction
- 00:42Determination of Mycobacterium tuberculosis (Mtb) Uptake by Macrophages: Multiplicity of Infection (MOI)
- 02:30Acid Fast Bacteria Staining
- 04:14Macrophage Infection
- 05:09Sample Collection
- 06:10Inoculating and Loading Instrument Culture Bottles
- 07:22Results:Mycobacterium tuberculosis Growth Quantification and Therapy Evaluation Using Automated Culture System
- 08:54Conclusion
Rapid and efficient quantification of intracellular M. tuberculosis growth is crucial for pursuing improved therapies against tuberculosis (TB). This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies.
Tags
Keywords:
Automated Culture SystemHost-directed TherapiesTuberculosisMycobacterium TuberculosisIntracellular GrowthFDA-approved DrugsRepurposingMycobacterial CultureEO Carrier T-cell CultureAttenuated Mycobacterial CultureH37RaCentrifugationRPMIResuspensionMycobacterial SuspensionGlass Chamber SlidesPhagocytosisPBSPFA
