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Journal
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Biochemistry
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测量CD95死亡诱导信号传导复合物的组成和该复合物中Procaspase-8的处理
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Biochemistry
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JoVE Journal
Biochemistry
Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex
Please note that all translations are automatically generated.
Click here for the English version.
测量CD95死亡诱导信号传导复合物的组成和该复合物中Procaspase-8的处理
DOI:
10.3791/62842-v
•
07:17 min
•
August 02, 2021
•
Laura K. Hillert-Richter
,
Inna N. Lavrik
1
Translational Inflammation Research, Center of Dynamic Systems
,
Otto von Guericke University Magdeburg
Chapters
00:05
Introduction
00:44
Preparing Cells and CD95L Stimulation
01:11
Cell Harvest and Lysis
02:29
Immunoprecipitation
03:28
Western Blot
04:21
Western Blot Detection
05:05
Analysis of CD95 DISC Formation in HeLa-CD95 cells, c-FLIPL-overexpressing HeLa-CD95 cells, and Primary T Cells
06:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,提出了一种实验工作流程,可以直接在死亡诱导信号复合物(DISC)上检测caspase-8处理并确定该复合物的组成。这种方法具有广泛的应用,从解开细胞死亡途径的分子机制到细胞凋亡网络的动态建模。
Tags
CD95
DISC
Apoptosis
Caspase-8
Immunoprecipitation
Cell Lysis
Protein Concentration
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