Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Medindo flux de substrato mitocondrial em células …
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Medindo flux de substrato mitocondrial em células o-permeabilizadas de permeabilized recombinante
Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
Journal (Biology)
Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
Establishment of a Density Gradient Centrifugation System to Isolate and Purify Bacterial Extracellular Vesicles (BEVs) from Human Faeces (Video) | JoVE
Journal (Biology)
Establishment of a Density Gradient Centrifugation System to Isolate and Purify Bacterial Extracellular Vesicles (BEVs) from Human Faeces (Video) | JoVE
 
Click here for the English version

Medindo flux de substrato mitocondrial em células o-permeabilizadas de permeabilized recombinante

Article DOI: 10.3791/62902-v • 06:17 min • August 13th, 2021
August 13th, 2021 •
1,2, 1,2, 1, 1, *2,3, *1
1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Neste trabalho, descrevemos um protocolo modificado para testar o flux de substrato respiratório mitocondrial usando perfringolysina recombinante O em combinação com respirometria à base de microplato. Com este protocolo, mostramos como a metformina afeta a respiração mitocondrial de duas linhas diferentes de células tumorais.

Tags

Biologia Edição 174 respirometria de microplacas mitocôndrias metformina células permeabilizadas perfringolysina O substratos mitocondriais
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter