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Aislamiento y cultivo de células epiteliales gingivales humanas primarias utilizando Y-27632
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1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine,Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology,Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology,Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery,Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School,Hangzhou Medical College
Chapters
- 00:00Introduction
- 00:49Gingival Tissue Pretreatment
- 01:13Digestion of Gingival Tissue
- 02:46Cell Passage
- 03:51Representative Results
- 06:18Conclusion
Aquí presentamos un método modificado para el aislamiento y cultivo de células epiteliales gingivales humanas mediante la adición del inhibidor de Rock, Y-27632, al método tradicional. Este método es más fácil, consume menos tiempo, mejora las propiedades de las células madre y produce un mayor número de células epiteliales de alto potencial tanto para el laboratorio como para aplicaciones clínicas.
