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Journal / Biochemistry / Immunopeptidomics: Isolering af mus og human mHC…
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Biochemistry

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JoVE Journal Biochemistry
Immunopeptidomics
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Immunopeptidomics: Isolering af mus og human mHC klasse I- og II-associerede peptider til massespektrometri analyse

Article DOI: 10.3791/63052-v • 09:32 min • October 15th, 2021
October 15th, 2021 •
*1, *1, 1, 1, 1,2
1CHU Sainte-Justine Research Center, 2Department of Pathology and Cellular Biology, Faculty of Medicine, Université de Montréal
* These authors contributed equally

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Her præsenterer vi en protokol til rensning af MHC klasse I og klasse II peptid komplekser fra mus og menneskelige cellelinjer giver høj kvalitet immunopeptidomics data. Protokollen fokuserer på prøveforberedelse ved hjælp af kommercielt tilgængelige antistoffer.

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